✦ LIBER ✦

Physical mapping of the mitochondrial genome of the cultivated mushroomAgaricus brunnescens (= A. bisporus)

✍ Scribed by William E. A. Hintz; James B. Anderson; Paul A. Horgen

Publisher: Springer-Verlag
Year: 1988
Tongue: English
Weight: 600 KB
Volume: 14
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00405852

No coin nor oath required. For personal study only.

✦ Synopsis

Mitochondrial (mt) DNA from the commercial mushroom Agaricus brunnescens Peck [= A. bisporus (Lange) Imbach] was purified by cesium chloride/bisbenzimide gradient centrifugation. A physical map of the mtDNA fragments produced by BamHI, EcoRI, and PvulI digestion was generated by Filter hybridizations with radiolabelled BamHI mtDNA probes. The A. brunnescens mtDNA was a circular molecule 136 kilo-basepairs (kbp) in length and contained an inverted repeat between 4.6 and 9.2 kbp in size. Orientational isomers of the mitochondrial genome were not detected. The positions of six genes were located on the A. brunnescens mtDNA map by heterologous hybridization. No coding function has yet been ascribed to the inverted repeat. The large rRNA gene was located on the smaller single copy region. The genes for cytochrome b, cytochrome oxidase (subunit III), ATPase (subunits 8 and 6) and the small rRNA were located on different regions of the larger single copy region.

📜 SIMILAR VOLUMES

Further characterization of a large inve

Further characterization of a large inverted repeat in the mitochondrial genomes ofAgaricus bisporus (=A. brunnescens)and related species

✍ Tianru Jin; P. A. Horgen 📂 Article 📅 1993 🏛 Springer-Verlag 🌐 English ⚖ 725 KB

The mitochondrial (mt) genome of Agaricus bisporus Ag50 (a heterokaryon) is a 136-kilobase (kb) circular molecule which contains a pair of large inverted repeats (IRs). Two large BAMHI fragments (B1 and B2) which contain the IR regions were further mapped. The repeated regions were determined to be

The mitochondrial genome of the aquatic

The mitochondrial genome of the aquatic phycomyceteAllomyces macrogynus. Physical mapping and mitochondrial DNA instability

✍ Bernhard Borkhardt; Terence A. Brown; Poul Thim; Lauritz W. Olson 📂 Article 📅 1988 🏛 Springer-Verlag 🌐 English ⚖ 884 KB

The mitochondrial genome of the basidiom

The mitochondrial genome of the basidiomyceteAgrocybe aegerita: molecular cloning, physical mapping and gene location

✍ Thierry Moulinier; Gérard Barroso; Jacques Labarère 📂 Article 📅 1992 🏛 Springer-Verlag 🌐 English ⚖ 831 KB

The mtDNA of a wild-type strain of Agrocybe aegerita was purified from mitochondria isolated by cellular fractionation. A representative library was constructed in E. coli by molecular cloning at the HindIII restriction site of pBR322. Southern hybridizations between total DNA of the fungal strain a

The genetic map of the mitochondrial gen

The genetic map of the mitochondrial genome in yeast

✍ Schweyen, R. J. ;Weiss-Brummer, B. ;Backhaus, B. ;Kaudewitz, F. 📂 Article 📅 1978 🏛 Springer 🌐 English ⚖ 900 KB

Purification and characterization of NAD

Purification and characterization of NADP-dependent glutamate dehydrogenase from the commercial mushroomAgaricus bisporus

✍ J. J. P. Baars; H. J. M. Op den Camp; A. H. A. M. Hoek; C. Drift; L. J. L. D. Gr 📂 Article 📅 1995 🏛 Springer 🌐 English ⚖ 727 KB

Physical and linkage mapping of the bovi

Physical and linkage mapping of the bovine genome with cosmids

✍ G. A. Hawkins; S. Solinas Toldo; M. D. Bishop; S. M. Kappes; R. Fries; Craig W. 📂 Article 📅 1995 🏛 Springer-Verlag 🌐 English ⚖ 896 KB