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The mitochondrial genome of the basidiomyceteAgrocybe aegerita: molecular cloning, physical mapping and gene location

✍ Scribed by Thierry Moulinier; Gérard Barroso; Jacques Labarère

Publisher
Springer-Verlag
Year
1992
Tongue
English
Weight
831 KB
Volume
21
Category
Article
ISSN
0172-8083

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✦ Synopsis

The mtDNA of a wild-type strain of Agrocybe aegerita was purified from mitochondria isolated by cellular fractionation. A representative library was constructed in E. coli by molecular cloning at the HindIII restriction site of pBR322. Southern hybridizations between total DNA of the fungal strain and cloned mitochondrial insert probes were used to establish the restriction map of the mtDNA molecule. Its size was assessed at about 80,500 bp. Four structural genes (for Cox 1, Cox 2, Atp 6, and Atp 8) were located on the map by heterologous hybridizations with oligonucleote probes specific for yeast mitochondrial genes. The location of the genes coding for the large and the small RNAs of the mitochondrial ribosome was determined by hybridization with the E. coli rrnB operon. A comparison of A. aegerita mtDNA organization with that of both phylogenetically close and distant fungi is discussed.

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