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Physical map of the COB region in mitochondrial DNA ofSaccharomyces cerevisiae

✍ Scribed by G. Grosch; C. Schmelzer; S. Mathews

Publisher
Springer-Verlag
Year
1981
Tongue
English
Weight
539 KB
Volume
3
Category
Article
ISSN
0172-8083

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✦ Synopsis

A physical map of the COB region in mtDNA of yeast has been established. This region harbours a split gene coding for apocytochrome b. It includes restriction sites of seven endonucleases (EcoRI, HindII, HindIII, HaeIII, HpaII, AluI and BamHI). Various mtDNA sequences of this region, retained in a series of genetically characterized rho(-) clones have been allocated to this map. The combination of this physical map with a genetic map of the rho(-) clones revealed that 1) cob(-) mutational sites spread over 8,400 bp, 2) mutations in sequences coding for apocytochrome b map in five distinct segments which are separated by intervening sequences with minimum lengths from 350 to 1,900 bp.

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