## Abstract Nutritional requirements of amphibian cells in culture were studied for the purpose of modifying a minimal medium in which frog cells could proliferate and which could be used for obtaining drugβresistant and auxotrophic variants. The serum, purine, CO~2~, and amino acid requirements fo
Photoreactivation of amphibian cells in culture
β Scribed by James D. Regan; John S. Cook; William H. Lee
- Publisher
- John Wiley and Sons
- Year
- 1968
- Tongue
- English
- Weight
- 308 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
β¦ Synopsis
Photoreactivation of growth and DNA synthesis in UV-irradiated cells and photoreactivatingenzyme activity of cell-free extracts can be demonstrated in a cell Iine derived from liver tissue of the African clawed toad, Xenopus Zaeuis. This cell line, A8W2, is a favorable system for the quantitative study of photoreactivation in vertebrates.
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## Abstract With Lβ15 as the base medium, drugβresistant variants were isolated from two amphibian tissue culture strains: the __Xenopus laevis__ A8 diploid cell line and the ICR 2A cell line of __Rana pipiens__. Four different classes of variants were obtained: (1) A8 cells resistant to chloramphe
## Abstract Xanthophores isolated from bullfrog tadpoles were successfully cloned. The rapid disappearance of the yellow pigmentβcontaining organelles (pterinosomes) occurred in proliferating xanthophores and many black granules were newly synthesized in the cells. The granules were electronmicrosc
d Gnioersity, Califomin SIX FIGURES Studies on photoreactivation, tlie reversal of injurious effects of ultraviolet radiation by illumination with visible light (Keliier, '49a), have been concerned mainly with viability and niutations (Kelner, '49a, b ; Dulbecco, '49 ; Kovick and Szilard, '49) but