Phospholipase D development during differentiation of human promyelocytic leukemic HL60 cells
β Scribed by Kenji Ohguchi; Shigeru Nakashima; Yoshinori Nozawa
- Book ID
- 117772567
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 283 KB
- Volume
- 1439
- Category
- Article
- ISSN
- 1388-1981
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## Abstract More than 80% of cells from a human promyelocytic leukemic cell line (HLβ60) possess the capacity for selfβrenewal as evidenced by their ability to form large primary colonies in semisolid medium and the presence within these colonies of cells capable of subsequent colony formation. Col
Human promyelocytic leukaemic cells, HLβ60, arrested in mitosis by nocodazole were released in the presence of 1Ξ±,25βdihydroxyvitamin D3 and thymidine or hydroxyurea. Cells moved from early G1 period to the G1/S boundary and differentiated. Furthermore, cells arrested at the G1/S boundary by double