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Phosphocreatine as a marker of contractile activity in single muscle fibres

✍ Scribed by Agnès Conjard; D. Pette

Book ID
106111765
Publisher
Springer
Year
1999
Tongue
English
Weight
47 KB
Volume
438
Category
Article
ISSN
0031-6768

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A sensitive method for the analysis o f ATP and phosphocreatine (PCr) in single human skeletal muscle fibres is described. Muscle tissue was freeze-dried and single fibres were dissected free w i t h the aid of low-power microscopy. The fibres were then extracted in trichloroacetic acid and neutrali