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Phosphocreatine and ATP content in human single muscle fibres before and after maximum dynamic exercise

✍ Scribed by C. Karatzaferi; A. de Haan; R. Ferguson; W. van Mechelen; A. Sargeant

Publisher: Springer
Year: 2001
Tongue: English
Weight: 116 KB
Volume: 442
Category: Article
ISSN: 0031-6768
DOI: 10.1007/s004240100552

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A sensitive method for the analysis o f ATP and phosphocreatine (PCr) in single human skeletal muscle fibres is described. Muscle tissue was freeze-dried and single fibres were dissected free w i t h the aid of low-power microscopy. The fibres were then extracted in trichloroacetic acid and neutrali