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Phenprocoumon Binding to Bovine and Human α1-Acid Glycoprotein

✍ Scribed by Jason Lauritsen; Edward C. Shane

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
92 KB
Volume
55
Category
Article
ISSN
0026-265X

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✦ Synopsis

The binding of the anticoagulant drug phenprocoumon to bovine and human a 1 -acid glycoprotein (AGP) was studied using a fluorescence titration technique. Phenprocoumon was found to have one moderate strength binding site on bovine AGP with n Å (0.78 { 0.04) and a Scatchard association constant, K Å (2.2 { 0.1) 1 10 4 M 01 . Phenprocoumon was bound more strongly to human AGP; a primary binding site was found with binding affinity, n 1 K 1 Å 4.6 1 10 5 M 01 , along with weaker secondary binding, n 2 K 2 Å 1.8 1 10 4 M 01 . ᭧ 1997 Academic Press

Methods

Racemic phenprocoumon was a gift of Hoffmann-La Roche, Inc. (Nutley, NJ). Bovine and human a 1 -AGP were obtained from the Sigma Chemical Company (St. Louis, MO). All other reagents were analytical grade. Solutions were buffered with

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