Phenoxyacetic acid induces glutathione-dependent detoxification and depletes the glutathione pool in Penicillium chrysogenum

✍ Scribed by Tamás Emri; Dr. István Pócsi; Attila Szentirmai

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 370 KB
• Volume: 37
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620370306

✦ Synopsis

Enzymes of the glutathione-dependent detoxification pathway (glutathione S-transferase and y-glutamyltranspeptidase) I) were induced, and the glutathione pool was completely depleted by phenoxyacetic acid in Penicillium chrysogenum mycelia incubated for 15 h in a culture medium containing lactose as a carbon source and sodium glutamate as a nitrogen source. A significant increase in both the oxidised glutathione concentrations and the glutathione reductase activities were also observed. 1 -Chloro-2,4-dinitrobenzene -a potent substrate and inducer of glutathione S-transferaseinitiated very similar physiological changes but no /3-lactam production could be detected in this case. When (NH4),HPO4 was used as a nitrogen source the penicillin biosynthesis was repressed and the induction of y-glutamyltranspeptidase by phenoxyacetic acid was hindered considerably.