Phenotypic characterization of ouabain-resistant Aedes albopictus cells

✍ Scribed by Steven J. Mento; Frank Malinoski; Victor Stollar

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 517 KB
• Volume: 101
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041010317

✦ Synopsis

Abstract

The phenotype of a ouabain‐resistant Aedes albopictus cell line has been partially characterized. Treatment of ouabain‐sensitive cells with 0.005–1.0 mM ouabain resulted in an 80% reduction in the uptake of ^86^rubidium (^86^Rb^+^), an ion with an affinity for the K^+^ pump binding site; ouabain‐resistant cells showed only a 40% reduction with 1.0 mM ouabain. When ouabain‐sensitive cells were incubated in the presence of ouabain (0.1 mM) for one and one‐half to three hours, the molar ratio of intracellular Na^+^/K^+^ rose from 0.2 to 4.2. In ouabain‐resistant cells, a similar treatment had very little effect. Based on [^3^H] ouabain‐binding studies, ouabain‐resistant cells were estimated to have 60% fewer binding sites per cell than ouabain‐sensitive cells. The spontaneous mutation rate from ouabain sensitivity to ouabain resistance was calculated to be 1–6 X 10^−8^ mutations/cell/generation, a value similar to that reported for mammalian cells at the analogous locus.