Phase separation in aqueous micellar solutions of nonionic surfactants for protein separation
β Scribed by Tohru Saitoh; Hirofumi Tani; Tamio Kamidate; Hiroto Watanabe
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 602 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0165-9936
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A water-soluble polymer such as polyethylene glycol (PEG), Dextran T-500 (Dx), or diethylaminoethyl-Dextran (DEAE-Dx) induced aqueous micellar solutions of octyl-β€-D-thioglucoside (OTG) to phase separation at 0Β°C. One of the two phases thus formed is a surfactantdepleted aqueous solution (aqueous ph
## Abstract A simple statisticalβmechanical theory, known as the randomβphase approximation, is applied to study liquidβliquid phase separations in solutions of globular proteins. Phase separation may be induced by addition of nonionic polymer or/and ordinary electrolytes. In this analysis, the osm
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