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pH-Induced conformational changes of DNA

✍ Scribed by L. Costantino; V. Vitagliano

Publisher
Wiley (John Wiley & Sons)
Year
1966
Tongue
English
Weight
330 KB
Volume
4
Category
Article
ISSN
0006-3525

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✦ Synopsis

The electrophoretic mobility of calf thymus DNA has been measured in aqueous buffered solutions as a function of pH. In the pH range 3.7-3.0, two electrophoretic species appear. The faster one migrates with the mobility of native DNA, the slower one migrates with a mobility close to that of thermally denatured DNA. The ratio of the two species varies with pH. Decreasing the pH increases the relative amount of the slower-moving component. These results may be interpreted by assuming that the DNA used in these experiments has a broad heterogeneity of base composition and that the conformational stability with respect t,o pH increases with increasing G + C content.

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