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Perturbations in Microtubule Mechanics from Tubulin Preparation

✍ Scribed by Hawkins, Taviare L.; Mirigian, Matthew; Li, Jingqiang; Yasar, M. Selcuk; Sackett, Dan L.; Sept, David; Ross, Jennifer L.


Book ID
113099459
Publisher
Springer
Year
2012
Tongue
English
Weight
665 KB
Volume
5
Category
Article
ISSN
1865-5025

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The presence of g-tubulin in microtubule preparations, obtained by disassembly/ assembly cycles at 0Β°C/37Β°C from the brain of several mammals, is demonstrated by immunoblotting with specific antibodies directed against three distinct regions of the protein. In contrast g-tubulin was absent from pure