Assay of penicillin acylase in organic m
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J. R. S. Alves; L. P. Fonseca; M. T. Ramalho; J. M. S. Cabral
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Article
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1995
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Springer-Verlag
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English
β 182 KB
The synthetic substrate 6-nitro-3-(phenylacetamido) benzoic acid (NIPAB) is an appropriate substrate for assaying penicillin acylase activity in reversed m&Bar systems of Aerosol -OT in &octane. Accumulation of 6-nitro-3-aminobenzoic acid (NABA) produced by the enzymatic hydrolysis of NIPAB, followe