๐”– Bobbio Scriptorium
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Assay of penicillin acylase in organic media

โœ Scribed by J. R. S. Alves; L. P. Fonseca; M. T. Ramalho; J. M. S. Cabral

Book ID
104648569
Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
182 KB
Volume
9
Category
Article
ISSN
0951-208X

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โœฆ Synopsis

The synthetic substrate 6-nitro-3-(phenylacetamido) benzoic acid (NIPAB) is an appropriate substrate for assaying penicillin acylase activity in reversed m&Bar systems of Aerosol -OT in &octane. Accumulation of 6-nitro-3-aminobenzoic acid (NABA) produced by the enzymatic hydrolysis of NIPAB, followed by the increase in absorbance at 405 nm, was linear at 4 to 20 mM for up to 30 minutes and 15 'C to4OT.

Abbreviations: PA, penicillin acylase (penicillin amidohydrolase EC 3.5.1.11); AOT, Aerosol OT (sodium bis-(2-ethylhexyl) sulfosuccinate); NIPAB, 6-nitro-3-@henylacetamido)-benzoic acid; NABA, 6nitro-3-aminobenzoic acid; BSA, bovine serum albumin.

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