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Peroxidase-catalyzed electrochemical assay of hydrogen peroxide: A ping–pong mechanism

✍ Scribed by F. Deyhimi; F. Nami

Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
182 KB
Volume
44
Category
Article
ISSN
0538-8066

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✦ Synopsis

In view of the great importance of determination of hydrogen peroxide in many scientific fields and industrial applications and the attractive operational simplicity of potentiometric approach for the enzymatic assay, the kinetics of horseradish peroxidase-catalyzed electrochemical assay of H 2 O 2 was studied in this work at 25 • C. All kinetic characteristics were determined by the double reciprocal Lineweaver-Burk and (primary and secondary) double reciprocal Hanes-Woolf plots. The results confirmed that the reaction follows a ping-pong mechanism. The Michaelis-Menten constants for H 2 O 2 and 4-fluorophenol were K H 2 O 2 m = 0.081 ± 0.001 mM and K 4-FP m = 0.185 ± 0.002 mM, respectively. The maximum rate was also estimated to be V max = 0.182 ± 0.002 mM min -1 (at 25 ± 0.05 • C).

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