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Peroxidase and hydrogen peroxide detection by a bioenergized method

✍ Scribed by Sonia M. De Toledo; Marcela Haun; Etelvino J.H. Bechara; Nelson Durán

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
236 KB
Volume
105
Category
Article
ISSN
0003-2697

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✦ Synopsis

Peroxidase activity and hydrogen peroxide concentrations were measured by a chemiluminescent method based upon the reduction of peroxidase Compounds I and II by both eosin and EDTA. Eosin excess present in the reaction mixture was excited during the reaction to its fluorescent state. This bioenergized method allows calculation of peroxidase concentration in the range of lo-l2 to lo-' M, and hydrogen peroxide concentration in the range of 10m9 to low5 M. This approach has been applied to the estimation of peroxidase activity in human red cell membranes and hydrogen peroxide formation in the peroxidasecatalyzed oxidation of glutathione.

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