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Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by digitonin

✍ Scribed by M.S. Joshi; L.R. Gowda; L.C. Katwa; S.G. Bhat

Publisher: Elsevier Science
Year: 1989
Tongue: English
Weight: 422 KB
Volume: 11
Category: Article
ISSN: 0141-0229
DOI: 10.1016/0141-0229(89)90140-3

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The yeast, Kluyveromyces fragilis was permeabilized to a number of low-molecular-weight substrates using digitonin. The activities of intracellular yeast enzymes, viz., alcohol dehydrogenase (ADH), beta-galactosidase, glucose-6-phosphate dehydrogenase, aspartase, and hexokinase were found to be much

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