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Permeability of reconstituted sarcoplasmic reticulum vesicles: Reconstitution of the K+, Na+ channel

✍ Scribed by Roger C. Young; Robert Allen; Gerhard Meissner


Book ID
115725314
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
645 KB
Volume
640
Category
Article
ISSN
0005-2736

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In order to study the conductances of the Sarcoplasmic Reticulum (SR) membrane, microsomal fractions from cardiac SR were isolated by differential and sucrose gradient centrifugations and fused into planar lipid bilayers (PLB) made of phospholipids. Using either KCl or K-gluconate solutions, a large