A method for monitoring enzymatic deacetylation processes of natural or artificial chitin substrates as well as N-acetylchitooligosaccharides by the direct determination of the acetate released is described. Furthermore, a new assay is presented for the determination of chitin deacetylase activity e
Peripheral enzymatic deacetylation of chitin and reprecipitated chitin particles
โ Scribed by Kyaw Nyein Aye; Renuka Karuppuswamy; Tangir Ahamed; Willem F. Stevens
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 291 KB
- Volume
- 97
- Category
- Article
- ISSN
- 0960-8524
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## Abstract Chitins produced via a conventional chemical route as well as from a new biological process were modified to increase the efficiency of enzymatic deacetylation reactions for the production of novel biological chitosan. These modified chitins were reacted for 24 h with extracellular fung
In the present article, chitin from crab shell was systematically deacetylated using a NaOH treatment with control of the reaction time. The degree of deacetylation, monitored using solid-state NMR, revealed that the reaction was pseudo-first order. Based on this, swollen and NaOH-saturated particle