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Peripheral enzymatic deacetylation of chitin and reprecipitated chitin particles

โœ Scribed by Kyaw Nyein Aye; Renuka Karuppuswamy; Tangir Ahamed; Willem F. Stevens


Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
291 KB
Volume
97
Category
Article
ISSN
0960-8524

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A method for monitoring enzymatic deacetylation processes of natural or artificial chitin substrates as well as N-acetylchitooligosaccharides by the direct determination of the acetate released is described. Furthermore, a new assay is presented for the determination of chitin deacetylase activity e

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In the present article, chitin from crab shell was systematically deacetylated using a NaOH treatment with control of the reaction time. The degree of deacetylation, monitored using solid-state NMR, revealed that the reaction was pseudo-first order. Based on this, swollen and NaOH-saturated particle