Performance of hollow-fiber flow field-flow fractionation in protein separation

✍ Scribed by Ilyong Park; Ki-Jung Paeng; Dukjin Kang; Myeong Hee Moon

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 342 KB
• Volume: 28
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200500125

✦ Synopsis

Abstract

Since hollow‐fiber flow field‐flow fractionation (HF FlFFF) utilizes a cylindrical channel made of a hollow‐fiber membrane, which is inexpensive and simple in channel assembly and thus disposable, interests are increasing as a potential separation device in cells, proteins, and macromolecules. In this study, performance of HF FlFFF of proteins is described by examining the influence of flow rate conditions and length of fiber (polyacrylonitrile or PAN in this work) on sample recovery as well as experimental plate heights. The interfiber reproducibility in terms of separation time and recovery was also studied. Experiments showed that sample recovery was consistent regardless of the length of fiber when the effective field strength (equivalent to the mean flow velocity at the fiber wall) and the channel void time were adjusted to be equivalent for channels of various fiber lengths. This supported that the majority of sample loss in HF FlFFF separation of apoferritin and their aggregates may occur before the migration process. It is finally demonstrated that HF FlFFF can be applied for characterizing the reduction in Stokes' size of low density lipoproteins from blood plasma samples obtained from patients having coronary artery disease and from healthy donors.