Peptide sequence determination of peptidosteroids on a single bead by electrospray ionization–mass spectroscopy
✍ Scribed by Gerd Vanhoenacker; Li Liu; Frederic Lynen; Annemieke Madder; Pierre De Clercq; Pat Sandra
- Book ID
- 101393955
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 368 KB
- Volume
- 25
- Category
- Article
- ISSN
- 1615-9306
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📜 SIMILAR VOLUMES
Electrospray ionization coupled to a quadrupole ion trap mass spectrometer is used to differentiate between the isobaric amino acids lysine and glutamine in sequence analysis of peptides. Collision-induced dissociation is used for fragmentation. Several isobaric peptides with one or more lysines or
An on-line method has been developed for the derivatization and coupled liquid chromatography (LC)/electrospray ionization (ESI) MS analysis of peptides at the femtomol level. Peptides are reacted with N-succinimidyl-2(3-pyridyl)acetate (SPA) in buffered aqueous medium at pH 7 following loading on a