✦ LIBER ✦

Differentiation of lysine/glutamine in peptide sequence analysis by electrospray ionization sequential mass spectrometry coupled with a quadrupole ion trap

✍ Scribed by U. Bahr; M. Karas; R. Kellner

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 248 KB
Volume: 12
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19981015)12:19<1382::aid-rcm327>3.0.co;2-j

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✦ Synopsis

Electrospray ionization coupled to a quadrupole ion trap mass spectrometer is used to differentiate between the isobaric amino acids lysine and glutamine in sequence analysis of peptides. Collision-induced dissociation is used for fragmentation. Several isobaric peptides with one or more lysines or glutamines at different positions were investigated. The ambiguous amino acid either in the peptide chain or at the C-or N-terminus can be clearly identified based on specific side chain fragment ions resulting from MS 3 or MS 4 of B-and Y@-fragment ions.