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Peptide Nucleic Acid-Assisted Topological Labeling of Duplex DNA

✍ Scribed by Vadim V. Demidov; Heiko Kuhn; Irina V. Lavrentieva-Smolina; Maxim D. Frank-Kamenetskii


Book ID
115639248
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
528 KB
Volume
23
Category
Article
ISSN
1046-2023

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A convenient small scale synthesis has been developed for obtaining peptide nucleic acid oligomers (PNAs). PNAs have been conjugated to a protein, staphylococcal nuclease, through disulfide exchange between a cysteine at the 3'-(carboxy) end of the PNA and an introduced cysteine on the surface of th