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Peptide methionine sulfoxide reductase: Biochemistry and physiological role

โœ Scribed by Nathan Brot; Herbert Weissbach


Book ID
101334905
Publisher
Wiley (John Wiley & Sons)
Year
2000
Tongue
English
Weight
385 KB
Volume
55
Category
Article
ISSN
0006-3525

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The methionine sulfoxide reductase (Msr) family is composed of two structurally unrelated classes of monomeric enzymes named MsrA and MsrB, which display opposite stereo-selectivities towards the sulfoxide function. MsrAs and MsrBs, characterized so far, share the same chemical mechanism implying su

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A simple and rapid assay has been developed to measure the enzymatic activity of peptide methionine sulfoxide reductase. The assay is based on the reduction of labeled N-acetylmethionine sulfoxide to N-acetvlmethionine. The N-acetylmethionine can be separated from the substrate by extraction into et