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Peptide chiral purity determination: hydrolysis in deuterated acid, derivatization with Marfey's reagent and analysis using high-performance liquid chromatography-electrospray ionization-mass spectrometry

✍ Scribed by David R. Goodlett; Perlette A. Abuaf; Peter A. Savage; Kevin A. Kowalski; Tarit K. Mukherjee; John W. Tolan; Nancy Corkum; Gideon Goldstein; Jonathan B. Crowther


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
787 KB
Volume
707
Category
Article
ISSN
1873-3778

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## Abstract A specific and relatively sensitive high‐performance liquid chromatography‐electrospray ionization mass spectrometry (HPLC‐ESI‐MS) was developed for the quantitative analysis of tiopronin enantiomers in rat plasma. The method is based on the derivatization of (+)‐tiopronin and (−)‐tiopr