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Peptide and protein loading into porous silicon wafers

✍ Scribed by Prestidge, C. A. ;Barnes, T. J. ;Mierczynska-Vasilev, A. ;Kempson, I. ;Peddie, F. ;Barnett, C.

Book ID
105364440
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
232 KB
Volume
205
Category
Article
ISSN
0031-8965

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✦ Synopsis

Abstract

The influence of peptide/protein size and hydrophobicity on the physical and chemical aspects of loading within porous silicon (pSi) wafer samples has been determined using Atomic Force Microscopy (AFM) and Time‐of‐Flight Secondary Ion Mass Spectroscopy (ToF‐SIMS). Both Gramicidin A (a small hydrophobic peptide) and Papain (a larger hydrophilic protein) were observed (ToF‐SIMS) to penetrate across the entire pSi layer, even at low loading levels. AFM surface imaging of pSi wafers during peptide/protein loading showed that surface roughness increased with Papain loading, but decreased with Gramicidin A loading. For Papain, the loading methodology was also found to influence loading efficiency. These differences indicate more pronounced surface adsorption of Papain. (Β© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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