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Penicillin acylase purification with the aid of pseudo-affinity chromatography

✍ Scribed by Valérie Fitton; Nicole Verdoni; Jean Sanchez; Xavier Santarelli

Book ID
117331151
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
91 KB
Volume
49
Category
Article
ISSN
0165-022X

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A continuous system for the recovery and purification of the penicillin acylase from crude extracts by recycling phenyl-Sepharose gel through three agitated vessels with disc filters of stainless steel is presented. The penicillin acylase present in the crude extract was adsorbed into the phenyl-Sep