Penicillin acylase immobilization depending on macromolecular crowding and catalysis in aqueous–organic medium

Chemoenzymatic one-pot synthesis of cefa

Chemoenzymatic one-pot synthesis of cefazolin from cephalosporin C in fully aqueous medium, involving three consecutive biotransformations catalyzed by D-aminoacid oxidase, glutaryl acylase and penicillin G acylase

✍ Roberto Fernández-Lafuente; JoséM. Guisán; Massimo Pregnolato; Marco Terreni 📂 Article 📅 1997 🏛 Elsevier Science 🌐 French ⚖ 190 KB

A new chemoenzymatic synthesis of Cefazolin through the correct assembly of three bioU'ansformations catalysed by D-aminoacid oxidase, glutaryl acylase and penicillin G acylase is described. This multienzymatic synthesis has been performed from the natural Cephalosporin C in fully aqueous medium wit