PCR Protocols (Methods in Molecular Biology)

✍ Scribed by John M.S. Bartlett, David Stirling

Publisher: Humana Press
Year: 2003
Tongue: English
Leaves: 517
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

📜 SIMILAR VOLUMES

PCR Protocols (Methods in Molecular Biol

📁 PCR Protocols (Methods in Molecular Biology)

✍ Daniel J. Park 📂 Library 📅 2010 🏛 Humana Press 🌐 English

Known for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications. PCR Protocols, Third Edition selects recently developed tools and tri

PCR Cloning Protocols (Methods in Molecu

📁 PCR Cloning Protocols (Methods in Molecular Biology)

✍ Bing-Yuan Chen, Harry W. Janes 📂 Library 📅 2002 🏛 Humana Press 🌐 English

Rutgers Univ., New Brunswick, NJ. Covers performing and optimizing PCR, cloning PCR products, mutagenesis and recombination, cloning unknown neighboring DNA, and library construction and screening. Features reproducible methods for isolating genes from all biological samples. Hardcover, comb-bound n

RT-PCR Protocols (Methods in Molecular B

📁 RT-PCR Protocols (Methods in Molecular Biology)

✍ Joseph O'Connell Joe O'Connell 📂 Library 📅 2010 🏛 Humana Press 🌐 English

PCR: Methods and Protocols (Methods in M

📁 PCR: Methods and Protocols (Methods in Molecular Biology, 2967)

✍ Lucília Domingues (editor) 📂 Library 📅 2023 🏛 Humana 🌐 English

<p><span>This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances

PCR: Methods and Protocols (Methods in M

📁 PCR: Methods and Protocols (Methods in Molecular Biology, 2967)

✍ Lucília Domingues (editor) 📂 Library 📅 2023 🏛 Humana 🌐 English

PCR Mutation Detection Protocols (Method

📁 PCR Mutation Detection Protocols (Methods in Molecular Biology)

✍ eds Theophilus, Bimal D. M.; Rapley, Ralph. 📂 Library 📅 2002 🏛 Humana Press, Totowa, N.J 🌐 English

<span>1Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, S