PCR Mutation Detection Protocols (Methods in Molecular Biology)

✍ Scribed by eds Theophilus, Bimal D. M.; Rapley, Ralph.

• Publisher: Humana Press, Totowa, N.J
• Year: 2002
• Tongue: English
• Leaves: 214
• Category: Library

✦ Synopsis

1Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.