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PCR-based plasmid vector construction for generation of recombinant viruses

โœ Scribed by Darelle Thomson; Greg Smith


Book ID
114139629
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
130 KB
Volume
94
Category
Article
ISSN
0166-0934

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A general method for the construction of
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A technique is presented by which mutations can be introduced into the Escherichia coli chromosome by gene replacement between the chromosome and a plasmid carrying the mutant gene. The segregational instability of plasmids in E. coli is used with high efficiency to isolate E. coli mutants. The meth