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PCR amplification and characterization of the intergenic spacer region of the ribosomal DNA in Pyrenophora graminea

✍ Scribed by Susanna Pecchia; Elisabetta Mercatelli; Giovanni Vannacci


Book ID
109322425
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
397 KB
Volume
166
Category
Article
ISSN
0378-1097

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DNA sequences from the intergenic spacer (IGS) region of the ribosomal operon were amplified by the polymerase chain reaction (PCR) technique using two primers derived from 16S and 23S rRNA conserved sequences. The PCR products, cleaved by 4 base cutting restriction enzymes, were used to differentia