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Identification ofArmillariaspecies from hokkaido by analysis of the intergenic spacer (IGS) region of ribosomal DNA using PCR-RFLP

✍ Scribed by Kazuhisa Terashima; Yasuhiro Kawashima; Joo Young Cha; Kiyoshi Miura


Publisher
Springer
Year
1998
Tongue
English
Weight
354 KB
Volume
39
Category
Article
ISSN
1340-3540

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DNA sequences from the intergenic spacer (IGS) region of the ribosomal operon were amplified by the polymerase chain reaction (PCR) technique using two primers derived from 16S and 23S rRNA conserved sequences. The PCR products, cleaved by 4 base cutting restriction enzymes, were used to differentia