Translocation of 14C-labelled assimilates down the petioles was studied in intact plants of Pelargonium zonale (L.) L'H6rit ex Ait. The central bundle of the petiole was dissected out and treated with solutions of various inhibitors. Whereas cytochalasin B had no effect on l~C-translocation, a disti
Patterns of translocation and metabolism of14C-labelled IAA in the phloem of Willow
โ Scribed by N. W. Lepp; A. J. Peel
- Publisher
- Springer-Verlag
- Year
- 1971
- Tongue
- English
- Weight
- 624 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0032-0935
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โฆ Synopsis
When 2-14-C-labelled IAA was applied to an isolated segment of Willow via a bark abrasion the pathway of transport of this compound was found to be located in the sieve elements as evidenced by the pattern of activity found in honeydew excreted by individuals of Tuberolachnus sMignus (Gmelin) feeding on the segment.
Further experiments have established that polarity of transport of 14C-IAA occurs in a basipetal direction when isolated segments of willow are orientated in a vertical position, with the morphological apex uppermost. No polarity was found when segments were orientated in a vertical position with the morphological base uppermost, or when the segments were orientated in a horizontal position. The metabolism of ItC-IAA was also studied with respect to orientation. It was shown that the conversion rate of IAA to IAA aspartate was influenced by the orientation of the segment. It is considered that this is not a direct effect of orientation on the rate of metabolism of IAA.
๐ SIMILAR VOLUMES
Isolated bark strips of willow were sealed on to polythene tubes having three compartments. Colonies of the aphid Tuberolachnus salignus Gmelin were established on the bark at each end of the strip. IAA or kinetin at a concentration of 1 0 -~ was applied to the cambial surface of the strip in one of
It is widely accepted that the floral stimulus produced in leaves is carried to the shoot apex passively in the phloem with the assimilate s~ream. Three kinds of evidence presented here suggest that the floral stimulus moves independently of the assimilates. Simultaneous determination of the veloci