Patterns of endogenous gangliosides and metabolic processing of exogenous gangliosides in cerebellar granule cells during differentiation in culture
β Scribed by Laura Riboni; Alessandro Prinetti; Marina Pitto; Guido Tettamanti
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 910 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0364-3190
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The expression of gangliosides of the lactosylceramide (LC) and of the gangliotetraosylceramide (GTC) series on the surface of cells from rat embryonic cerebellar tissue was investigated by double-color indirect immunofluorescence. GD3 was assumed to be representative of LC and was detected using a
A study has been made of the association properties of the two GM 1 ganglioside molecular species GM 1-Cl8 and GM 1-C20 (containing Cl8 and C20 long chain bases, respectively) to rat cerebellar granule cells in culture. Both gangliosides recognized, to the same extent, and associated with them to gi
The effect of ethanol on ganglioside metabolism was assessed in cultured rat cerebellar granule cells. Cells were incubated in the presence of tritiated serine or galactose, and the synthesis of radioactive gangliosides was followed. The rate of de novo biosynthesis of gangliosides labeled in the ol
In order to investigate the molecular mechanism underlying synaptogenesis, we examined the dynamics and stability of syntaxin 1A and SNAP-25 in cultured cerebellar granule cells. In neurons cultured for less than 5 days in vitro (DIV), syntaxin was highly expressed with a half-life of ΟΎ48 hours. SNA