Rat cerebellar granule cells in culture associate and metabolize differently exogenous GM1 ganglioside molecular species containing a C18 or C20 long chain base
✍ Scribed by Manuela Valsecchi; Vanna Chigorno; Sandro Sonnino; Guido Tettamanti
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 435 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0009-3084
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✦ Synopsis
A study has been made of the association properties of the two GM 1 ganglioside molecular species GM 1-Cl8 and GM 1-C20 (containing Cl8 and C20 long chain bases, respectively) to rat cerebellar granule cells in culture. Both gangliosides recognized, to the same extent, and associated with them to give a form of association, the trypsin-labile form. This form was removed by treatment with trypsin enzyme. Both gangliosides associated stably with the cells to become components of the cell membranes. Although similar amounts of the two gangliosides entered the cells, being then metabolized, the time course of the association was different for the two gangliosides: after 15 h of ganglioside-ceU incubation the amount of GMI-CI8 inserted into the cell membrane was 2.43 times higher than that of GMI-C20.