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Patterning Proteins on Surfaces of Cross-Sectioned Multilayer Polymer Films

✍ Scribed by Chun Zhang; Scott M. Husson; Douglas E. Hirt

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
333 KB
Volume
27
Category
Article
ISSN
1022-1336

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✦ Synopsis

Abstract

Summary: A new approach is introduced to create submicrometer patterned surfaces using multilayer polymer films that contain alternating layers of two polymers, linear low‐density polyethylene (LLDPE) and ethylene‐co‐(acrylic acid) copolymer (EAA). Patterned templates have been prepared by microtoming the multilayer molded sheets. Regionally confined chemical functionality is confirmed by grafting an amine‐terminated biotin and adsorbing streptavidin specifically on the alternating layers of EAA.

Fluorescence micrograph of the Alexa488‐streptadivin patterned polymer surface.

magnified imageFluorescence micrograph of the Alexa488‐streptadivin patterned polymer surface.

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