Parvoviruses are inefficient in inducing interferon-β, tumor necrosis factor-α, or interleukin-6 in mammalian cells

✍ Scribed by Jörg R. Schlehofer; Martin Rentrop; Daniela N. Männel

• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 748 KB
• Volume: 181
• Category: Article
• ISSN: 0300-8584
• DOI: 10.1007/bf00202055

✦ Synopsis

To investigate a possible role of cytokines in parvovirus-mediated suppression of tumorigenesis, we tested in cell culture whether parvoviruses are able to induce interferon (IFN)-~, tumor necrosis factor (TNF)-a or interleukin-6 (IL-6). Infection of rodent or human cells with the parvoviruses minute virus of mice (MVM), H-1 or adeno-associated virus (AAV) types 2 or 5 failed to induce expression of the luciferase or J3-galactosidase reporter genes transfected into these cells as constructs containing an IFN-J3 promoter. Parvoviruses did weakly induce synthesis of TNF-~ and of IL-6 in cell culture and could slightly enhance synthesis of these cytokines when induced by other agents. These in vitro data suggest that the rather unspecific tumor-suppressive properties of parvoviruses are unlikely to be attributable to stimulation of the synthesis of IFN, TNF or IL-6.