Parvovirus diagnostics and vaccine production in insect cells
✍ Scribed by José Ignacio Casal
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 780 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0920-9069
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Recombinant human parvovirus B19 virus-like particles (VLPs), a candidate vaccine, were produced using the insect cell (Sf-9)-baculovirus (AcNPV) expression sys- tem. The synthesis and assembly of the particles in Sf-9 cells are directed by double infections with one recombinant virus (bacVP1) expre
A model for immunoglobulin G (IgG) production in the baculovirus-insect cell system was developed that incorporates polypeptide synthesis, oligomer assembly, protein aggregation, and protein secretion. In addition, the capacity of a chaperone to protect heavy and light chain polypeptides from protei