✦ LIBER ✦

Partition techniques for isolation and fractionation of urinary glycosaminoglycans

✍ Scribed by Robert E. Hurst; Gary C. Jennings; Andrew E. Lorincz

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 795 KB
Volume: 79
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90424-9

No coin nor oath required. For personal study only.

✦ Synopsis

A method for the isolation and a second method for the fractionation of urinary glycosaminoglycans (GAG) are presented. Both methods are based upon the partition of complexes formed between GAG and quaternary ammonium salts in two-phase systems of butanol and aqueous salt. The method for isolation permits the complete recovery, in one step, of the total urinary GAG in high purity. The other method presents a technique by which urinary GAG can be fractionated with high resolution. Each of the mucopolysaccharidosis types examined afforded a distinctive fmctionation pattern, and several structural differences in the GAG excreted in these disorders were noted.

📜 SIMILAR VOLUMES

A study of procedures for isolation and

A study of procedures for isolation and fractionation of plant cell walls

✍ R.C. Bean; L. Ordin 📂 Article 📅 1961 🏛 Elsevier Science 🌐 English ⚖ 862 KB

A detergent-citric acid technique for is

A detergent-citric acid technique for isolating nuclear and cytoplasmic fractions containing undegraded RNA from cells of Xenopus laevis

✍ Leo Miller 📂 Article 📅 1979 🏛 Elsevier Science 🌐 English ⚖ 722 KB

A simple technique is described for fractionating cells ofxenopus laevis using Triton X-100 and citric acid. Cells are treated for 1 min with a hypotonic buffer containing 0.5% Triton X-100 and RNAase inhibitors. The cells are then disrupted by adding citric acid to a final concentration of 1% for 2

Evaluation of four isolation techniques

Evaluation of four isolation techniques for honey aroma compounds

✍ Eleftherios Alissandrakis; Petros A Tarantilis; Paschalis C Harizanis; Moschos P 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 155 KB

Fractionation of plant material. III.—Tw

Fractionation of plant material. III.—Two schemes for chemical fractionation of fresh leaves, having special applicability for isolation of the bulk protein

✍ A. C. Jennings; A. Pusztai; R. L. M. Synge; W. B. Watt 📂 Article 📅 1968 🏛 John Wiley and Sons 🌐 English ⚖ 993 KB

Isolation of Acute-Phase Proteins from P

Isolation of Acute-Phase Proteins from Plasma for Determination of Fractional Synthesis Rates by a Stable Isotope Tracer Technique

✍ Farook Jahoor; Bhattiprolu Sivakumar; Melanie Del Rosario; Elizabeth M. Frazer 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 114 KB

Comparison of various extraction techniq

Comparison of various extraction techniques for isolation and determination of isoflavonoids in plants

✍ Tomáš Bajer; Martin Adam; Lubomír Galla; Karel Ventura 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 703 KB

## Abstract In the present paper, the following extraction techniques have been used for extracting isoflavonoids from the species __Matricaria recutita, Rosmarinus officinalis, Foeniculum vulgare__, and __Agrimonia eupatoria__ L.: supercritical fluid extraction (SFE), pressurized fluid extraction,