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Comparison of various extraction techniques for isolation and determination of isoflavonoids in plants

✍ Scribed by Tomáš Bajer; Martin Adam; Lubomír Galla; Karel Ventura

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
703 KB
Volume
30
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

In the present paper, the following extraction techniques have been used for extracting isoflavonoids from the species Matricaria recutita, Rosmarinus officinalis, Foeniculum vulgare, and Agrimonia eupatoria L.: supercritical fluid extraction (SFE), pressurized fluid extraction, matrix solid phase dispersion, ultrasonic extraction in an ultrasonic bath (USE) and by means of an ultrasonic homogeniser (HOM), extraction by means of Soxhlet apparatus (SOX), and solid phase extraction. Experimental optimization of all techniques has been carried out using a soybean flour. Subsequent analyses of the extracts were carried out by liquid chromatography with UV detection. The maximum yields of daidzein and genistein were obtained by extraction with the SOX, USE, and HOM techniques. The maximum yields of apigenin and biochanin A from herb samples were obtained by SFE.

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