Partition behaviour of horseradish peroxidase isoenzymes in aqueous two-phase poly(ethyleneglycol)/phosphate systems

✍ Scribed by María V. Miranda; Osvaldo Cascone

• Book ID: 104646470
• Publisher: Springer-Verlag
• Year: 1994
• Tongue: English
• Weight: 388 KB
• Volume: 8
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00155421

✦ Synopsis

Partition of purified horseradish peroxidase isoenzymes in aqueous two-phase systems was not affected by pH changes, but tie-line length and NaCl addition greatly increased the partition coefficient of ail three isoenzymes, the former having more influence than the latter. In all systems, K were higher for acidic than those for neutral and basic isoenzymcs. and K for basic were the lowest.