The e β ect of anionic (sodium butylbenzene sulfonate, sodium butylmonoglycol sulfate), cationic (tetrabutyl ammonium bromide), nonionic(Tween 20) and amphoteric (proline) surface active additives on the partitioning of proteins and enzymes, such as BSA, lysozyme, glucose oxidase and b-lactoglobulin,
The partition of sodium phosphate and sodium chloride in aqueous dextran poly(ethylene glycol) two-phase systems
β Scribed by Stephan Bamberger; Geoffrey V.F Seaman; Julie A Brown; Donald E Brooks
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 495 KB
- Volume
- 99
- Category
- Article
- ISSN
- 0021-9797
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A model for the prediction of protein partition coefficients in aqueous two-phase systems has been developed. This model accounts for both charge-independent and electrostatic effects. The determination of nonelectrostatic effects was based on the model of Eiteman and Gainer for uncharged solutes wh
Human albumin and alpha-fetoprotein are partitioned in an aqueous two-phase system composed of 10% (w/w) Dextran and 7.5% (w/w) poly(ethylene glycol). When a small amount of poly(ethylene glycol) is replaced by Cibacron Blue F3G-A-liganded poly(ethylene glycol) the partition coefficient, K, of album