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Partial purification of DNA-dependent RNA polymerases from small dense nuclei of mouse brain

โœ Scribed by K. Bradley Stokes; Nancy M. Lee; Horace H. Loh


Book ID
115774282
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
244 KB
Volume
27
Category
Article
ISSN
0006-2952

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Purification of DNA-dependent RNA polyme
โœ Grant A. Bitter ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 807 KB

A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, a