Purification and partial characterizatio
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Rabindra Randhawa; Ramji L. Khandelwal
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Article
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1990
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Springer
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English
β 721 KB
Glycogen synthase kinase-3 (GSK-3) was purified from rabbit liver to homogeneity by ultracentrifugation, ion-exchange chromatography on DEAE-cellulose, Cellulose phosphate, CM-Sephadex and Fast Protein Liquid Chromatography (FPLC) on Mono-S column. The enzyme was purified approximately 20,000 fold w