A DNA helicase activity was detected in extracts of purified chloroplasts from the SB-1 cell line of Glycine max and partially purified by column chromatography on DEAE cellulose, phosphocellulose, and single-stranded DNA cellulose. The chloroplast helicase has a DNA-dependent ATPase activity, and i
Chloroplast adenylate kinase from tobacco. Purification and partial characterization
โ Scribed by Uwe Schlattner; Edgar Wagner; Hubert Greppin; Marc Bonzon
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 458 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0031-9422
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Glycogen synthase kinase-3 (GSK-3) was purified from rabbit liver to homogeneity by ultracentrifugation, ion-exchange chromatography on DEAE-cellulose, Cellulose phosphate, CM-Sephadex and Fast Protein Liquid Chromatography (FPLC) on Mono-S column. The enzyme was purified approximately 20,000 fold w
Acetate kinase (EC 2.7.2.1), an enzyme involved in the wasteful production of acetate during conversion of cellulose to ethanol by Clostridlum thermocellum, was purified 144-fold. The enzyme has an Mr of 84 kD by non-denaturing gradient gel electrophoresis, and an Mr of 46 kD when estimated with a d