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Partial purification and characterization of a DNA helicase from chloroplasts ofGlycine max

โœ Scribed by Gordon C. Cannon; Sabine Heinhorst

Publisher
Springer
Year
1990
Tongue
English
Weight
800 KB
Volume
15
Category
Article
ISSN
0167-4412

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โœฆ Synopsis

A DNA helicase activity was detected in extracts of purified chloroplasts from the SB-1 cell line of Glycine max and partially purified by column chromatography on DEAE cellulose, phosphocellulose, and single-stranded DNA cellulose. The chloroplast helicase has a DNA-dependent ATPase activity, and its strand displacement activity is strictly dependent upon the presence of a nucleoside triphosphate and Mg 2 * or Mn 2 +. Strand displacement activity does not require a free unannealed single-strand or replication fork-like structure.

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