✦ LIBER ✦
Partial purification and characterization of the soluble DNA polymerase (polymerase-α) from seedlings ofPisum sativumL.
✍ Scribed by Christine Stevens; John A. Bryant
- Publisher
- Springer-Verlag
- Year
- 1978
- Tongue
- English
- Weight
- 536 KB
- Volume
- 138
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
✦ Synopsis
Soluble DNA polymerase has been extracted from pea seedlings and partially purified by chromatography on columns of DEAE-cellulose or DEAE-Sephadex. The enzyme elutes from DEAEcellulose as a single peak, but is fractionated into three peaks, SI, SIa and SII by DEAE-Sephadex chromatography. SIa and SII may be derived from SI by freeze-thaw treatment or by treatment with (NH4)2 SO~. The ion and pH requirements and the sensitivity to N-ethyl maleimide of the pea seedling soluble DNA polymerase are similar to those of the DNA polymerase-c~ from vertebrates.