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Partial purification and characterization of pentalenene synthase

✍ Scribed by David E. Cane; Christopher Pargellis


Book ID
115707328
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
839 KB
Volume
254
Category
Article
ISSN
0003-9861

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Acetohydroxyacid synthase from Claviceps
✍ Dr. Walter Maier; Rajesh Luthra; Detlef GrΓΆger πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 466 KB

An acetohydroxyacid synthase (EC 4.1.3.18) which synthesizes a-acetolactate from pyruvate has been isolated from two different CIaviceps purpurea strains. A purification of about 142-fold was achieved by ammonium sulfate fractionation and the use of Sepharose 6B and DEAE-Sepharose CL-6B columns. The